Yes, you just set up each bank account.  If you use bank deposits in Sage 50, also set up a 'cash and cheques on hand' account.

One will be the 'default', but the other can be manually selected on any screen where payments or deposits are made.

The 'universal construction' example file books are set up with multiple bank accounts.

In addition to what Randy has said, the numbering sequence is available on an account by account basis under Setup, Reports & Forms, Cheques, pick the bank account and at the bottom is the next cheque number field.  This can and will eventually be different for each bank account even if you start both at the same number and will show on the Payments module Source number field when you switch bank accounts.

It's a good idea to start with a different set of nbrs for each bank account if you want to be able to know at a glance which chq came out of which bank account - one bank acct starts with Chq nbrs 1000 and the other bank account starts with chq nbrs 5000 - depending on how many chqs per year you go thru.

Smithco has a good point about the numbering.   Also on the subject of document numbering, if you start invoices, Sales Orders, Purchase Orders, Cheques, Receipts, etc. with high numbers you will also find it easier to identify documents - ie 706032 is a cheque, 606032 is a purchase order, 206032 is an invoice, etc.

And turn off 'use next available number' under Setup | Settings | Company | Forms, unless your cheque printer is in a locked room and never prints anything but cheques for one account.  If the cheques get out of sequence, or if you have a misprint, or print onto white paper, Sage 50 will:

- wait until you print, then over-ride to the next cheque number regardless of what you see or type into the screen,

- print the wrong number on the cheque,

- then taunt you with its cleverness and an 'OK' button.

I totally agree with RandyW re the 'turning off 'use next available nbr' - no doubt this is one of Sage's "new improved' (totally useless frustrating) function.